Journal: SICOT-J

Article Title: Prevalence of low bone mineral density in robotic-assisted TKA candidates: insights from quantitative CT analysis

doi: 10.1051/sicotj/2025048

Figure Lengend Snippet: Automatic selection of regions of interest (ROIs) for QCT analysis. Axial CT slice processed with the CTXA Hip module (QCT Pro, Mindways). The green boxes indicate the automated placement of the hip ROI and the calibration phantom, which are used to extract bone mineral density values for osteoporosis assessment.

Article Snippet: Axial CT slice processed with the CTXA Hip module (QCT Pro, Mindways).

Techniques: Selection

Journal: Toxins

Article Title: Detection of Extremely Low Level Ciguatoxins through Monitoring of Lithium Adduct Ions by Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

doi: 10.3390/toxins16040170

Figure Lengend Snippet: LC-MS/MS (MRM mode) chromatograms of the NIHS-CTX-Mix comprised of CTX1B (1.00 ng/mL), 2.3-dihydroxyCTX3C (2.80 ng/mL), 52- epi -54-deoxyCTX1B (1.45 ng/mL), 51-hydroxy-CTX3C (2.70 ng/mL), 54-deoxyCTX1B (1.15 ng/mL), 49- epi CTX3C (1.20 ng/mL), CTX3C (1.05 ng/mL), CTX4A (1.50 ng/mL), and CTX4B (1.10 ng/mL). Analyses were carried out with acetonitrile-based mobile phases containing ( A ) Na + and ( B ) Li + and MRM transitions were set as [M+Na] + > [M+Na] + and [M+Li] + > [M+Li] + , respectively. The LC-MS/MS conditions are shown in . ( A ) [M+Na] + > [M+Na] + : CTX1B m / z 1133.60 > 1133.60, 2.3-dihydroxyCTX3C m / z 1079.60 > 1079.60, 52- epi -54-deoxyCTX1B and 54-deoxyCTX1B m / z 1117.60 > 1117.60, 51-hydroxyCTX3C m / z 1061.60 > 1061.60, 49- epi CTX-3C and CTX-3C m / z 1045.60 > 1045.60, CTX4A and CTX4B m / z 1083.60 > 1083.60. ( B ) [M+Li] + > [M+Li] + : CTX1B m / z 1117.60 > 1117.60, 2.3-dihydroxyCTX3C m / z 1063.60 > 1063.60, 52- epi -54-deoxyCTX1B and 54-deoxyCTX1B m / z 1101.60 > 1101.60, 51-hydroxyCTX3C m / z 1045.60 > 1045.60, 49- epi CTX3C, and CTX3C m / z 1029.60 > 1029.60, CTX4A and CTX4B m / z 1067.60 > 1067.60.

Article Snippet: The CTX mix solution (JFRL-CTX-Mix) was prepared from five CTX reference materials: CTX1B, 52- epi -54-deoxyCTX1B, 51-hydroxyCTX3C, CTX3C, and CTX4A (JFRL-CTX-RMs), supplied by JFRL (Japan Food Research Laboratories, Tokyo, Japan).

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Toxins

Article Title: Detection of Extremely Low Level Ciguatoxins through Monitoring of Lithium Adduct Ions by Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

doi: 10.3390/toxins16040170

Figure Lengend Snippet: LC-MS/MS chromatograms at the lowest calibration point of CTX analogs in the JFRL-CTX-Mix. ( a ) CTX1B: 0.0022 ng/mL, ( b ) 52- epi -54-deoxyCTX1B: 0.0060 ng/mL, ( c ) 51-hydroxyCTX3C: 0.0055 ng/mL, ( d ) CTX3C: 0.0050 ng/mL, and ( e ) CTX4A: 0.0055 ng/mL.

Article Snippet: The CTX mix solution (JFRL-CTX-Mix) was prepared from five CTX reference materials: CTX1B, 52- epi -54-deoxyCTX1B, 51-hydroxyCTX3C, CTX3C, and CTX4A (JFRL-CTX-RMs), supplied by JFRL (Japan Food Research Laboratories, Tokyo, Japan).

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Toxins

Article Title: Detection of Extremely Low Level Ciguatoxins through Monitoring of Lithium Adduct Ions by Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

doi: 10.3390/toxins16040170

Figure Lengend Snippet: The calibration curves of the CTX analogs. ( a ) CTX1B (0.0022–0.110 ng/mL), ( b ) 52- epi -54-deoxyCTX1B (0.0060–0.120 ng/mL), ( c ) 51-hydroxyCTX3C (0.0055–0.110 ng/mL), ( d ) CTX3C (0.0050–0.100 ng/mL), ( e ) CTX4A (0.0055–0.110 ng/mL).

Article Snippet: The CTX mix solution (JFRL-CTX-Mix) was prepared from five CTX reference materials: CTX1B, 52- epi -54-deoxyCTX1B, 51-hydroxyCTX3C, CTX3C, and CTX4A (JFRL-CTX-RMs), supplied by JFRL (Japan Food Research Laboratories, Tokyo, Japan).

Techniques:

Journal: Toxins

Article Title: Detection of Extremely Low Level Ciguatoxins through Monitoring of Lithium Adduct Ions by Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

doi: 10.3390/toxins16040170

Figure Lengend Snippet: Evaluation results for the lowest point on the calibration curve (LOQ).

Article Snippet: The CTX mix solution (JFRL-CTX-Mix) was prepared from five CTX reference materials: CTX1B, 52- epi -54-deoxyCTX1B, 51-hydroxyCTX3C, CTX3C, and CTX4A (JFRL-CTX-RMs), supplied by JFRL (Japan Food Research Laboratories, Tokyo, Japan).

Techniques:

Journal: Advanced Science

Article Title: DEL‐1 is an Endogenous Senolytic Protein that Inhibits Senescence‐Associated Bone Loss

doi: 10.1002/advs.202509263

Figure Lengend Snippet: DEL‐1 deficiency exacerbates chemotherapy‐induced bone loss. Doxorubicin was administered to 9‐week‐old WT and Del1 KO mice at a dose of 5 mg kg −1 per week via intraperitoneal injection for 4 weeks. After 4 weeks, femurs from both WT and Del1 KO mice were extracted and analyzed using microcomputed tomography to assess bone loss. A) Schematic representation of the experimental timeline for doxorubicin administration. B) Representative µCT images of bone microarchitecture at the sagittal plane (left) and the mid‐diaphysis of the femur (middle). Quantification of µCT‐derived cortical thickness (Ct.Th(mm), right). C) Representative µCT images of bone microarchitecture at the distal femoral metaphysis (left). Quantification of µCT–derived trabecular bone parameters, including bone volume fraction (Tb.BV/TV(%)), trabecular number (Tb.N(mm −1 )), trabecular thickness (Tb.Th(mm)), and trabecular space (Tb.Sp(mm)) (right). D,E) Fasting serum levels of bone formation marker osteocalcin (D), bone resorption markers collagen type I cross‐linked C‐telopeptide (CTX1) and tartrate‐resistant acid phosphatase 5b (TRAcP 5b) (E). F) Histology, immunostaining, and TRAP staining of femurs. Representative images of H&E staining (scale bar, 500 µm), TRAP‐positive osteoclasts (purple; scale bar, 100 µm), and osteocalcin (OCN)‐positive osteoblasts (green; scale bar, 100 µm) in a boxed area on the H&E staining panels. The nuclei were counterstained using DAPI (blue). G) Expression of senescence‐related genes, p21, p53, Il6, Cxcl2, Tgfβ1, and p16 , in the bone marrow cells assessed by qPCR. Gapdh was used for normalization of mRNA expression and the gene expression of WT mice was assigned an average value of 1. H) Levels of IL‐6, TGFβ1, and CXCL2 in bone marrow fluid, measured by ELISA. I) Representative fluorescence images of p21⁺ cells (purple) in the bone marrow compartment of femoral bone sections. The nuclei were counterstained with DAPI (blue). Scale bar, 100 µm. Data are means ± SD (B,C: n =4‐5 mice/group; D,E: n =5 mice/group; G: n =3–4 mice/group; H: n =6–7 mice/group). * p < 0.05; ** p < 0.01; *** p <0.001; NS, not significant. Unpaired Student's t ‐test, except for “CTX1” in panel E (Mann‐Whitney U test).

Article Snippet: Serum levels of CTX1 (catalogue no. AC‐06F1, Immunodiagnostic Systems), TRAcP 5b (catalogue no. SB‐TR103, Immunodiagnostic Systems), and osteocalcin (catalogue no. EEL003, Thermo Fisher Scientific), were measured using ELISA kits, following the manufacturer's protocol.

Techniques: Injection, Tomography, Derivative Assay, Marker, Immunostaining, Staining, Expressing, Gene Expression, Enzyme-linked Immunosorbent Assay, Fluorescence, MANN-WHITNEY